发明名称 |
ANTIBODY-BONDABLE PROTEIN HAVING IMPROVED DISSOCIATION CHARACTERISTIC IN SLIGHTLY ACIDIC REGION, AND ANTIBODY SCAVENGER |
摘要 |
<P>PROBLEM TO BE SOLVED: To provide an improved protein of the outer membrane domain of a cell of protein G, having bondability to Fc region of immunoglobulin in a slightly acidic region and/or bondability to Fab region thereof reduced compared to that of the outer membrane domain of the cell of a wild-type protein G without damaging the high antibody-bonding activity in a neutral region. <P>SOLUTION: The modified protein is obtained using an amino acid residue present within the distance of 6.5A from the Fc region as a mutation targeting site in the three-dimensional structural coordinate data of a complex obtained by bonding the outer membrane domain of the cell of the protein G with the Fc region of the immunoglobulin G, and having ≥40% exposed surface area rate, or an amino acid residue present in the Fab region or within 4A distance therefrom in the three-dimensional structural coordination data of a complex obtained by bonding the outer membrane domain of the cell of the protein G with the Fab region of the immunoglobulin G, and having ≥40% exposed surface area rate, and subjecting the amino acid residue to substitution with another amino acid residue. The substitutions can be combined. <P>COPYRIGHT: (C)2010,JPO&INPIT |
申请公布号 |
JP2009297018(A) |
申请公布日期 |
2009.12.24 |
申请号 |
JP20090007538 |
申请日期 |
2009.01.16 |
申请人 |
NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL & TECHNOLOGY |
发明人 |
HONDA MASAYA;WATANABE HIDEKI;MATSUMARU HIROYUKI;FENG YANWEN |
分类号 |
C12N15/09;C07K14/315;C07K17/00;C12N1/15;C12N1/19;C12N1/21;C12N5/10 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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