NUKLEINSÄUREAMPLIFIKATION AUF BASIS VON KÜGELCHENEMULSION
摘要
This invention relates to methods of generating single stranded DNA libraries for use in amplification and sequencing reactions. In various aspects, the disclosed methods include: fragmenting DNA; polishing the fragments' ends; lighting the fragments to universal adapters; performing strand displacement and extension of the nicked fragments; purifying the double-stranded libation products; capturing the double-stranded libation products onto a solid support; and isolating single stranded DNA library fragments, and binding these fragments to another solid support.
申请公布号
DE602004024034(D1)
申请公布日期
2009.12.24
申请号
DE20046024034T
申请日期
2004.01.28
申请人
454 CORP.
发明人
BERKA, JAN;CHEN, YI-JU;LEAMON, JOHN H.;LEFKOWITZ, STEVEN;LOHMAN, KENTON;MAKHIJANI, VINOD;SARKIS, GARY J.;ROTHBERG, JONATHAN;WEINER, MICHAEL;SRINIVASAN, MAITHREYAN