METHOD FOR THE PURIFICATION OF BIOLOGICAL MACROMOLECULES

摘要

Disclosed are methods and devices for electrophoretic separation of free biomolecules from molecular complexes comprising biomolecules of interest during which inhomogeneity in the molecular complexes is masked. The methods can include loading a sample which contains free biomolecules and the complexes that include biomolecules of interest to a proximal end of an electrophoresis gel. The sample is electrophoresed for a period of time sufficient for the free biomolecules to elute off a distal end of the gel while the molecular complexes that include the biomolecules of interest are retained in the electrophoresis gel, thereby separating the molecular complexes from the free biomolecules. The direction of electrophoresis is reversed and the sample is electrophoresed for a period of time sufficient for the molecular complexes of bound biomolecules of interest to elute from the proximal end of the electrophoresis gel.