DETERMINATION OF DISTRIBUTION

摘要

A method for determining the occurrence of an analyte subpopulation of heteroforms of a substance (= S) in a liquid sample. The method comprises as its main characteristic features the step of: (i) providing a flow path which a) comprises an outlet part and an inlet part, b) comprises a capture zone (CZ) containing a solid phase exhibiting an immobilized analyte specific binder (B) [= affinity counterpart to the substance] which is capable of affinity binding to S with an affinity that differs for the various heteroforms of S, and c) permits capillary suction from the outlet part for driving a liquid flow through CZ, (ii) flowing said liquid sample containing S in the downstream direction through CZ while S is captured by said binder B in CZ, (iii) determining the distribution of S along the flow direction in CZ by measuring the relative amount of S in at least one subzonei of CZ, and (iv) determining the occurrence of the analyte subpopulation based on the distribution determined in step (iii).