发明名称 |
Process for selecting polyhydroxyalkanoate (PHA) producing micro-organisms |
摘要 |
The invention relates to a process for selecting a polyhydroxyalkanoate (PHA) producing micro-organism from a natural source comprising a variety of micro-organisms, comprising steps of preparing a fermentation broth comprising the natural source and nutrients in water; creating and maintaining aerobic conditions in the fermentation broth; applying a feast-famine cycle comprising a feast phase and a famine phase, wherein the feast-famine cycle is started with feeding a carbon source to the fermentation broth, nutrients are fed in such amounts to the fermentation broth that the carbon source becomes limiting for the growth of the micro-organisms during the famine phase; the feast-famine cycle has a cycle time of at least 8 hours, and the feeding of the carbon source is performed during a small initial fraction of the feast famine cycle time; withdrawing a part of the micro-organisms during and/or at the end of the feast-famine cycle (referred to as solids withdrawal step); and repeating the feast-famine-cycle and the solids withdrawal step, such that an average solids retention time (SRT) of less than 4 days results. The invention also relates to a mixed culture or isolated microorganism which has an activity of producing PHA, obtainable by the said selection process, use thereof in a process for producing a PHA, and the resulting PHA containing microorganism. |
申请公布号 |
EP2135954(A1) |
申请公布日期 |
2009.12.23 |
申请号 |
EP20080158500 |
申请日期 |
2008.06.18 |
申请人 |
TECHNISCHE UNIVERSITEIT DELFT |
发明人 |
LOOSDRECHT VAN, MARINUS CORNELIS MARIA;KLEEREBEZEM, ROBBERT;MUYZER, GERARD;JIANG, YANG;JOHNSON, KATJA |
分类号 |
C12P7/62 |
主分类号 |
C12P7/62 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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