| 摘要 |
SNP(single nucleotide polymorphism) markers are provided to discriminate Hanwoo(Korean cattle) from importing cattle accurately, improve the mixed circulation structure of Hanwoo, milk cow, domestic cattle and importing cattle, and maintain and promote production foundation of Hanwoo by protecting gene sources of Hanwoo and identifying classes of Hanwoo. An SNP marker for discriminating Hanwoo from importing cattle is selected from the nucleotide sequences of SEQ ID NO:1 to SEQ ID NO:70. A method for discriminating Hanwoo from importing cattle comprises the steps of: (i) extracting DNA from a cattle to be examined; (ii) amplifying the DNA containing any one base mutation site of SEQ ID NO:1 to SEQ ID NO:70 or a complementary sequence therewith; (iii) synthesizing a primer complementary with the nucleotide sequence of any one base mutation site of SEQ ID NO:1 to SEQ ID NO:70 or with the complementary sequence therewith containing one base site adjacent thereto; (iv) determining the base kind used in the reaction of step (iii) by using a sequencer; and (v) comparing the base kind determined in step (iv) with a control.
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