发明名称 |
RECOMBINANT DEAMIDATED GLIADIN ANTIGEN |
摘要 |
The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.
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申请公布号 |
US2009311727(A1) |
申请公布日期 |
2009.12.17 |
申请号 |
US20090425605 |
申请日期 |
2009.04.17 |
申请人 |
BIO-RAD LABORATORIES, INC. |
发明人 |
WATKINS MICHAEL I.;MARR GREGORY A.;YANG XIAOYUN;BRUEHL RICHARD;SHAN DAMING;COLEMAN PATRICK F. |
分类号 |
G01N33/53;C07H21/04;C07K14/415;C12N5/10;C12N15/63 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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