SINGLE VECTOR FOR THE EXPRESSION OF HUMAN DECAY ACCELERATING FACTOR(DAF) AND THE FUSED PROTEIN OF THE FUCTIONAL DOMAIN OF HUMAN TISSUE FACTOR PATHWAY INHIBITOR(TFPI) AND THE ADHESIVE DOMAIN OF CLUSTER DIFFERENTIATION FACTOR 4(CD4) AND PROCASS MAKING THERE OF

摘要

PURPOSE: A combination vector for developing pig for bio organ is provided to suppress necrosis of pig tissue and cells and prevent blood coagulation. CONSTITUTION: A pLPC-pLTR/pCMVIE-hDAF-pSV40-GFP-pCMVIE-hTFPI/hCD4 vector suppress complement reaction, blood coagulation by expressing human decay accelerating factor(hDAF) and human tissue factor pathway inhibitor hTFPI/hCD4(human cluster differentiation factor 4) fusion protein. The hDAF, hTFPI and CD4 is obtained by extracting RNA from human blood, performing RT-PCR(reverse transcription-polymerase chain reaction) to synthesize cDNA of hDAF, hTFPI, and hCD4, inserting restriction enzyme recognition site and promoter sequence and performing PCR to obtain dsDNA. A transformed pig cell is made by transforming pig cells with pLPC-pLTR/pCMVIE-hDAF-pSV40-GFP-pCMVIE-hTFPI/hCD4 vector.