发明名称 |
Enzyme-catalyzed metal deposition for the enhanced in situ detection of immunohistochemical epitopes and nucleic acid sequences |
摘要 |
The invention is directed to novel compositions of matter and methods of detecting in situ an immunohistochemical epitope or nucleic acid sequence of interest in a biological sample comprising binding an enzyme-labeled conjugate molecule to the epitope or sequence of interest in the presence of a redox-inactive reductive species and a soluble metal ion, thereby facilitating the reduction of the metal ion to a metal atom at or about the point where the enzyme is anchored. Novel phosphate derivatives of reducing agents are described that when exposed to a phosphatase are activated to their reducing form, thereby reducing metal ions to insoluble metal.
|
申请公布号 |
US7632652(B2) |
申请公布日期 |
2009.12.15 |
申请号 |
US20040877919 |
申请日期 |
2004.06.24 |
申请人 |
VENTANA MEDICAL SYSTEMS, INC. |
发明人 |
BIENIARZ CHRISTOPHER;KERNAG CASEY A.;KOSMEDER JEROME W.;RODGERS PAULA;WONG JENNIFER |
分类号 |
C12Q1/42;C07F9/09;C07F9/655;C07H15/20;C07H15/24;C07H15/26;C12Q1/44;C12Q1/54;C12Q1/68;G01N33/53;G01N33/543;G01N33/567;G01N33/58 |
主分类号 |
C12Q1/42 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|