发明名称 |
PREPARATIVE PURIFICATION PROCESS FOR HUMAN FURIN |
摘要 |
Recombinant truncated human furin was expressed in CHO cells and concentrated approximately 50-fold by ultrafiltration and diafiltration. The concentrate was purified by column chromatography on Capto-MMC(TM) resulting in a 30-50 fold purification factor and a yield of at least 60%. The at least 20% pure preparation obtained after Capto-MMC(TM) chromatography had already a purification degree allowing on-column maturation of pro-VWF. Then an additional Arginine Sepharose chromatography purification was carried out. This two column process for purification of truncated human furin resulted in an almost pure furin preparation with a specific activity of approximately 290,000 U furin/mg protein and a yield of about 50%.
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申请公布号 |
US2009304669(A1) |
申请公布日期 |
2009.12.10 |
申请号 |
US20080124390 |
申请日期 |
2008.05.21 |
申请人 |
BAXTER INTERNATIONAL INC.;BAXTER HEALTHCARE S.A. |
发明人 |
MATTHIESSEN PETER;ROMEDER-FINGER STEFAN;TURECEK PETER;SCHWARZ HANS-PETER |
分类号 |
A61K38/48;A61P43/00;C12N9/48 |
主分类号 |
A61K38/48 |
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