Method For Expressing Polypeptides In Eukaryotic Cells Using Alternative Splicing

摘要

This invention relates to an expression cassette for expressing polypeptides in eukaryotic cells using alternative splicing. The expression cassette comprises in 5' to 3' downstream direction: a promoter; a sequence transcribed in a 5' untranslated region (5'UTR); a donor splice site; an intron; a first acceptor splice site; a first cistron encoding a first polypeptide; a second acceptor splice site; a second cistron encoding a second polypeptide; an internal ribosome entry site (IRES) operably linked to a selection marker; and a sequence transcribed in a 3'' untranslated region (3'UTR) including a polyadenylation signal, wherein the polyadenylation signal is unique.