发明名称 |
TRANSFORMATION OF ALGAL CELLS |
摘要 |
<p>Exemplary methods include a method for transforming an algal cell by preparing a transformation construct, preparing a particle for bombarding the algal cell, adhering the transformation construct to the particle, bombarding the algal cell with the particle, and growing the algal cell into a colony. The transformation construct is replicated within a nuclear genome of the algal cell and the growing of the algal cell is in a nutrient medium. Another exemplary method may include a method for genetically modifying an algal cell, by adding nucleic acid to the algal cell while the algal cell is suspended in a solution of low conductivity, introducing the nucleic acid into the algal cell by application of an electrical pulse resulting in a transformed algal cell, and selecting a colony that includes the transformed algal cell.</p> |
申请公布号 |
WO2009149465(A1) |
申请公布日期 |
2009.12.10 |
申请号 |
WO2009US46650 |
申请日期 |
2009.06.08 |
申请人 |
AURORA BIOFUELS, INC.;VICK, BERTRAND;KILIAN, OLIVER |
发明人 |
VICK, BERTRAND;KILIAN, OLIVER |
分类号 |
C12N15/82 |
主分类号 |
C12N15/82 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|