The present invention relates to a method for preparing erythropoietin, wherein culture supernatant of erythropoietin-producing eukaryotic cells containing erythropoietin are subjected to the following steps: a) Removing the cell components; and b) treating the product from a) to the following chromatography steps in the sequence indicated i) reversed phase chromatography; ii) anion exchange chromatography; iii) hydroxyapatite chromatography.
申请公布号
CA2727042(A1)
申请公布日期
2009.12.10
申请号
CA20092727042
申请日期
2009.05.28
申请人
EVONIK DEGUSSA GMBH
发明人
WIENAND, WOLFGANG;KUNZ, FRANZ-RUDOLF;REICHERT, DIETMAR;EUL, WILFRIED;HANKO, RUDOLF;BIRR, CHRISTIAN;SINGHOFER-WOWRA, MONIKA;SCHOPOHL-KOENIG, DAGMAR;FABER, LARS