METHOD FOR EFFICIENTLY AMPLIFYING ABNORMAL PRION PROTEIN DERIVED FROM SHEEP SCRAPIE

摘要

The object aims to provide a method for efficiently amplifying an abnormal prion protein (PrPSc) derived from sheep scrapie, to find a sheep infected by scrapie at an early stage to eradicate the propagation of prion disease, and to enable the development of a method for inactivating prion and the evaluation of the inactivation of prion at an early stage.  Thus, disclosed is a method for efficiently amplifying an abnormal prion protein (PrPSc) derived from sheep scrapie by a PMCA (protein misfolding cyclic amplification) method in which a PrPSc derived from sheep scrapie is amplified by using a normal prion protein (PrPC) as a source and the PrPSc as a seed and carrying out the mixing/culture and the ultrasonic treatment of both the PrPC and the PrPSc repeatedly, wherein a PrPC from a different animal is used as the PrPC.