RECOMBINANT E. COLI PRODUCING RECOMBINANT HUMAN VARIATION INTERFERON-beta PROTEIN REMOVED AMINO TERMINAL METHIONINE AND PREPARATION METHOD THEREOF

摘要

PURPOSE: A method for preparing a recombinant E.coli which produces a recombinant human mutant interferon-beta is provided to omit methionine removal process and massively protrude the recombinant protein. CONSTITUTION: A method for preparing a recombinant E.coli(KACC 95075P) which produces a recombinant human mutant interferon-beta protein comprises: a step of inserting the recombinant human mutant interferone-beta(rHu IFN-βSer17, IFN-β 1b) gene into pET21a(+) vector to produce a recombinant expression vector pET21a(+)IFN-βSer17; a step of inserting methionine aminopeptidase gene into pSTV29 vector to obtain recombinant expression vector pSTV29Map; and a step of transforming pET21a(+)IFN-βSer17 and pSTV29Map in E.coli.