发明名称 METHOD OF DETECTING BINDING SITE TO DNA-BINDING PROTEIN IN DNA
摘要 <P>PROBLEM TO BE SOLVED: To provide a method whereby a region containing a binding site of a DNA capable of binding to a DNA-binding protein in the DNA can simply and quickly be detected. Ž<P>SOLUTION: Provided is the method characterized by comprising: preparing a first liquid mixture containing a fluorescent labeled DNA-binding protein and a first DNA known as being bound by the above protein and a second liquid mixture containing a second DNA and a fluorescent labeled DNA-binding protein; determining the presence or absence of a difference in motion speed between the DNA-binding protein in the first liquid mixture and the DNA-binding protein in the second liquid mixture on the basis of the fluorescence intensities of the first and second liquid mixtures; and detecting the region containing the binding site of the DNA on the basis of the results. Ž<P>COPYRIGHT: (C)2010,JPO&INPIT Ž
申请公布号 JP2009278866(A) 申请公布日期 2009.12.03
申请号 JP20060238819 申请日期 2006.09.04
申请人 OLYMPUS CORP 发明人 AIDA KAZUFUMI
分类号 C12Q1/68;C12N15/09;C12Q1/34;G01N21/78 主分类号 C12Q1/68
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