发明名称 |
NOVEL NUCLEIC ACID PROBES, METHOD FOR DETERMINING NUCLEIC ACIDS BY USING THE PROBES, AND METHOD FOR ANALYZING DATA OBTAINED BY THE METHOD |
摘要 |
(1) A novel nucleic acid probe comprising a single- stranded oligonucleotide labeled with a fluorescent dye such that, when the nucleic acid probe is hybridized with a target nucleic acid, the intensity of fluorescence in a hybridization reaction system decreases after a hybridization reaction, and (2) a novel nucleic acid probe comprising a single-stranded oligonucleotide labeled with a fluorescent dye and a quencher substance such that the intensity of fluorescence in a hybridization reaction system increases after a hybridization reaction, the latter oligonucleotide forming no stem-loop structure between bases at positions where the oligonucleotide is labeled with the fluorescent dye and the quencher substance, respectively; various determination kits and DNA chips making use of these probes; method for determining nucleic acids, polymorphous analysis methods and real-time quantitative PCR methods, all of which make use of the determination kits or DNA chips.
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申请公布号 |
CA2383939(C) |
申请公布日期 |
2009.12.01 |
申请号 |
CA20012383939 |
申请日期 |
2001.06.27 |
申请人 |
KANKYO ENGINEERING CO., LTD.;NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCEAND TECHNOLOGY |
发明人 |
KURANE, RYUICHIRO;KURATA, SHINYA;KANAGAWA, TAKAHIRO;TORIMURA, MASAKI;KAMAGATA, YOICHI;YAMADA, KAZUTAKA;YOKOMAKU, TOYOKAZU |
分类号 |
C12Q1/68;C07H21/00;C12M1/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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