| 摘要 |
ANTIBOPIES AGAINST INSULIN-LIKE GROWTH FACTOR I RECEPTOR. Arm USES THEREOF Abstract An antibody binding to IGF-IR and inhibiting the binding of IGF-I and IGF-ll to IGF-IR which is characterized in that said antibody is a) is of IgGl isotype,b) shows a ratio of IC50 values of inhibition of the binding of' IGF-I- to IGF-IR to the inhibition of binding,of IGF-ll to IGF-IR of-l:3 to 3:1,c) inhibits for at least 80% at a concentration of 5 nM IGF-IR phosphorylation in a cellular phosphorylation assay using 3T3 cells providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0.5% heat inactivated fetal calf serum (FCS) when compared to, such an assay without said antibody, andd) shows no IGF-IR stimulating activity measured as IGF-IR phosphorylation at a concentration of 10 uM in a cellular phosphorylation assay using 3T3 cells providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0.5% heat inactivated fetal calf serum (FCS) when compared to such an assay without said antibody has improved properties in antitumor therapy.
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