| 摘要 |
<p>Disclosed is a method for initiating a new cultured line of human pluripotent stem cells without the use of feeder cells or conditioned medium, the method comprising the step of plating cells from a blastocyst onto a matrix in a medium including albumin, minerals, vitamins, amino acids, glucose, a fibroblast growth factor, gamma amino butyric acid, pipecolic acid, lithium lipids, a transferrin or a transferrin substitute and insulin or an insulin substitute in sufficient amounts to originate and maintain a new proliferating stem cell line in an undifferentiated state.</p> |
