Inactivation of a pathogen in a sample by a treatment with formalin and UV light

摘要

A method for inactivating a virus contained in a sample, the method comprising the steps of: (i) treating the sample with an effective concentration of formalin, and (ii) subjecting the sample with an effective dose of UV light in a flow-through apparatus, and (iii) determining the residual infectivity of the inactivated virus in the sample using a mammalian cell safety test comprising the steps of: a) Inoculating a mammalian cell culture with a purified inactivated enveloped virus. b) Incubating the cell culture. c) Harvesting the cell culture and inoculating a second mammalian cell culture with the harvest. d) Incubating the second cell culture. e) Harvesting the second cell culture and testing the harvest for hemagglutinin activity; Wherein step (i) is performed prior to step (ii) or vice versa.