Enteric-coated glucosinolates and beta-thioglucosidases

摘要

Disclosed is a non-therapeutic method of converting glucosinolate to isothiocyanate in the small intestine comprising orally administering to a subject an enteric-coated chemoprotectant precursor composition, said method comprising: (1) enteric-coated beta-thioglucosidase particles; and (2) enteric-coated glucosinolate particles, wherein beta-thioglucosidase and glucosinolate are released from the enteric-coated beta-thioglucosidase particles and enteric-coated glucosinolate particles, respectively, in the small intestine where beta-thioglucosidase converts glucosinolate to isothiocyanate. Also disclosed is a non-therapeutic method of converting glucosinolate to isothiocyanate in the small intestine comprising orally administering to a subject an enteric-coated capsule containing a chemoprotectant precursor composition comprising: (1) beta-thioglucosidase particles; and (2) glucosinolate particles, wherein beta-thioglucosidase and glucosinolate are released from the beta-thioglucosidase particles and glucosinolate particles, respectively, in the small intestine where beta-thioglucosidase converts glucosinolate to isothiocyanate. Also disclosed is an enteric-coated chemoprotectant precursor composition comprising: (1) about 0.5 to about 50 weight percent of glucosinolate particles; (2) about 0.5 to about 50 weight percent of beta-thioglucosidase particles; and (3) about 1 to about 99 weight percent of a composition comprising a coating excipient and optionally a diluent, wherein the glucosinolate or beta-thioglucosidase particles is encapsulated with an enteric coating. Also disclosed is an enteric-coated capsule comprising a chemoprotectant precursor mixture, the chemoprotectant mixture comprising uncoated beta-thioglucosidase particles and uncoated glucosinolate particles in a ratio of about 1:100 to about 100:1, wherein the mixture is contained within an enteric-coated capsule.