| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for refining a recombinant human FSH in high yield and high purity from a culture fluid of recombinant human FSH-producing cells using only steps without use of any organic solvents. <P>SOLUTION: The method for producing a recombinant human FSH comprises the steps of: (a) culturing recombinant human FSH-producing mammalian cells in a serum-free medium and secreting a recombinant human FSH in the culture fluid, (b) preparing a culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography to retrieve a human FSH-active fraction, (d) subjecting the fraction to dye affinity column chromatography to retrieve a human FSH-active fraction, (e) subjecting the fraction to hydrophobic column chromatography to retrieve a human FSH-active fraction, and then (f) subjecting the fraction to gel filtration column chromatography to retrieve the final recombinant human FSH-active fraction. <P>COPYRIGHT: (C)2010,JPO&INPIT |
