2-F MODIFIED RNA INTERFERENCE AGENTS

摘要

This invention relates to a method of modulating the expression of a target gene in an organism comprising administering an iRNA agent, wherein the iRNA comprises at least one 2'-deoxy-2'-fluoro (2'-F) nucleotide in the antisense strand and at least one modified nucleotide in the sense strand. The invention also relates to compositions comprising a single-stranded oligonucleotide that contains at least one 2'-deoxy-2'-fluoro (2'-F) nucleotide. siRNA molecule containing these oligonucleotides have decreased immunogenicity.