发明名称 VERFAHREN ZUR EINFÜHRUNG EINER NUKLEINSÄURE
摘要 The present invention relates to a method of introducing nucleic acids into cells by electroporation, comprising the step (A) of loading nucleic acids to the surface of an electrode; the step (B) of adhering cells on the obtained nucleic acid-loaded electrode surface; and the step (C) of applying electric pulses to the adhering cells. According to this method, not only efficient introduction of a gene into cells but also gene introduction at desirable timing and at desirable sites can be performed without damaging the adhering cells.
申请公布号 DE602004023631(D1) 申请公布日期 2009.11.26
申请号 DE20046023631T 申请日期 2004.08.20
申请人 KYOTO UNIVERSITY 发明人 IWATA, HIROO;KATO, KOICHI;YAMAUCHI, FUMIO
分类号 C12N15/09;C12M1/00;C12N5/10;C12N15/87;C25B11/04 主分类号 C12N15/09
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