发明名称 |
VERFAHREN ZUR EINFÜHRUNG EINER NUKLEINSÄURE |
摘要 |
The present invention relates to a method of introducing nucleic acids into cells by electroporation, comprising the step (A) of loading nucleic acids to the surface of an electrode; the step (B) of adhering cells on the obtained nucleic acid-loaded electrode surface; and the step (C) of applying electric pulses to the adhering cells. According to this method, not only efficient introduction of a gene into cells but also gene introduction at desirable timing and at desirable sites can be performed without damaging the adhering cells. |
申请公布号 |
DE602004023631(D1) |
申请公布日期 |
2009.11.26 |
申请号 |
DE20046023631T |
申请日期 |
2004.08.20 |
申请人 |
KYOTO UNIVERSITY |
发明人 |
IWATA, HIROO;KATO, KOICHI;YAMAUCHI, FUMIO |
分类号 |
C12N15/09;C12M1/00;C12N5/10;C12N15/87;C25B11/04 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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