ELECTROPORATION OF ADHERENT CELLS WITH AN ARRAY OF CLOSELY SPACED ELECTRODES

摘要

Adherent cells and other membranous structures that are immobilized on a solid surface are transfected by electroporation in which the electric field is produced by a array of closely spaced electrodes positioned above the surface. Each electrode is substantially smaller in at least one lateral dimension than the dimensions of a single cell, and the electrodes in each pair are spaced apart by distances selected such that a maximum of one cell will reside within the field produced by each pair, and the distance of the electrodes above the surface to which the cells are adherent is small enough to place the cell within the resulting electric field and yet great enough to avoid contact of the electrodes with the cell membrane.