| 摘要 |
A method and kit for precipitating protein from an aqueous solution consists of a quaternary ammonium cationic surfactant and a chaotropic salt, used in combination to reversibly precipitate proteins from the aqueous solution. Precipitation separates proteins that are entrapped, non-specifically, in the quaternary ammonium cationic surfactant:chaotropic salt complex as a pellet. The precipitated protein pellet is purified further by washing with an organic solvent to remove the surfactant:chaotrope complex, leaving behind pure, concentrated proteins that can be washed to purify further the protein and/or re-dissolved in whatever buffer is most suited for any successive procedure, such as analysis.
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