发明名称 |
PROCESS, VECTORS AND ENGINEERED CELL LINES FOR ENHANCED LARGE-SCALE TRANSFECTION |
摘要 |
<p>Processes vectors and engineered cell lines for large-scale transfection and protein production in mammalian cells, especially Chinese Hamster Ovary (CHO) cells are described in which transfection efficiencies are realized through the use of a single vector system, the use of functional oriP sequences in all plasmids, the use of codon- optimized Epstein-Barr virus nuclear antigen-1 (EBNA1 ) constructs the use of a fusion protein between a truncated Epstein-Barr virus nuclear antigenen-1c (EBNA1c) protein and a herpes simplex virus protein VP16, the use of a 40 kDa fully deacetylated poly(ethylenimine) as a transfection reagent, the use of co-expression of a fibroblast growth factor (FGF) and/or the use of protein kinase B to potentiate heterologous gene expression enhancement by valproic acid (VPA).</p> |
申请公布号 |
WO2009137911(A1) |
申请公布日期 |
2009.11.19 |
申请号 |
WO2009CA00263 |
申请日期 |
2009.03.09 |
申请人 |
NATIONAL RESEARCH COUNSIL OF CANADA;DUROCHER, YVES;LOIGNON, MARTIN |
发明人 |
DUROCHER, YVES;LOIGNON, MARTIN |
分类号 |
C12N15/85;C07K14/035;C07K14/05;C07K19/00 |
主分类号 |
C12N15/85 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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