METHOD FOR INTRODUCTION OF GENE INTO YEAST CELL, AND VECTOR FOR THE METHOD

摘要

Disclosed is a method for introducing a foreign gene into a yeast cell having no auxotrophic marker.  Specifically disclosed is a method for imparting a target auxotrophy to a yeast cell and introducing a gene to be expressed into the yeast cell.  The method comprises the step of transforming a yeast cell with an expression cassette for a gene to be expressed, a cassette for a yeast selection marker and a fragment containing two homologously recombined fragments which are homologous to regions located on both sides of a target auxotrophy-controlling gene in a predetermined array.  According to the method, a target auxotrophy-controlling gene can be deleted from a yeast cell, a gene to be expressed can be introduced into the yeast cell, and a yeast selection marker can be removed from the tansformed yeast cell.