| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for measuring a more accurate fluorescence value, in carrying out a real time PCR while making a PCR reaction solution flow in a flow channel and measuring the amount of a target nucleic acid by fluorescence, which measures a more accurate fluorescence value. SOLUTION: The method for measuring a target nucleic acid with which a PCR reaction solution containing at least a target nucleic acid is made to flow in a flow channel, the PCR reaction solution flowing in the flow channel is provided with a temperature cycle and subjected to PCR amplification, the fluorescence of the PCR reaction solution is measured for each temperature cycle and the target nucleic acid in the PCR reaction solution is measured includes making a solution for correction flow in the flow channel, providing the solution with the temperature cycle, measuring the fluorescence of the solution for correction as a background fluorescence for each temperature cycle and correcting the fluorescence value of each temperature cycle of the PCR reaction solution by using the fluorescence value of the corresponding background fluorescence. COPYRIGHT: (C)2010,JPO&INPIT |
