HETEROLOGOUS EXPRESSION OF FUNGAL CELLOBIOHYDROLASES IN YEAST

摘要

The present invention provides for heterologous expression of polypeptides encoded by wild-type and codon-optimized variants of cbhl and/or cbh2 from the fungal organisms Talaromyces emersonii (T. emersonii), Humicola grisea (H. grisea), Thermoascus aurantiacus (T. aurantiacus), and Trichoderma reesei (T. reesei) in host cells, such as the yeast Saccharomyces cerevisiae. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed cellobiohydrolases. Thus, such genes and expression systems are useful for efficient and cost- effective consolidated bioprocessing systems.