| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a highly sensitive immunological detection method whereby an allergen (for example, milk allergen, egg albumen allergen, wheat allergen, buckwheat allergen or peanut allergen) contained in a food can be detected in any state (i.e., either denatured or undenatured), and to provide a detection kit to be used therein. <P>SOLUTION: The method for detecting an allergen uses two or more monoclonal antibodies capable of recognizing milk allergen in the undenatured and denatured states, egg albumen allergen in the undenatured and denatured states, wheat allergen in the undenatured and denatured states, buckwheat allergen in the undenatured and denatured states, or peanut allergen in the undenatured and denatured states, wherein use is made of, as an indication,αs1αs1 casein which is the major protein inαs1 casein,β-lactoglobulin which is the major protein in whey, ovalbumin and ovomucoid which are the major proteins in egg albumen, gliadin which is the major protein in wheat, proteins of 24 kDa and 76 kDa in molecular weight which are the major proteins in buckwheat, or Ara h1 which is the major protein in peanut. <P>COPYRIGHT: (C)2010,JPO&INPIT</p> |
