CELL POPULATIONS FOR POLYPEPTIDE ANALYSIS AND USES OF SAME

摘要

Nucleic acid construct systems are disclosed. The constructs comprise: (i) a first nucleic acid construct comprising a first nucleic acid sequence encoding a first reporter polypeptide linked to an additional nucleic acid sequence capable of inserting the first nucleic acid construct into a genome of a host cell such that an endogenous polypeptide covalently attached to the first reporter polypeptide is expressed in the cell; and (ii) a second nucleic acid construct comprising a second nucleic acid sequence encoding a second reporter polypeptide, linked to an additional nucleic acid sequence capable of inserting in a non-directed manner the second nucleic acid construct into a genome of a host cell such that an endogenous polypeptide covalently attached to the second reporter polypeptide is expressed in the cell, wherein the first reporter polypeptide and the second reporter polypeptide are distinguishable. Cells and cell populations comprising same as well as methods of generating same are also disclosed. In addition, use of the novel construct systems are disclosed for identifying target agents are also disclosed.