发明名称 |
Method for production of recombinant human FSH |
摘要 |
<p>Disclosed is a method for production of recombinant human FSH in high yield and a high purity. The method comprises the steps of: (a) culturing recombinant human FSH-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) dye affinity column chromatography, (e) hydrophobic column chromatography, and (f) gel filtration column chromatography to collect recombinant human FSH-active fractions, in the order.</p> |
申请公布号 |
EP2119727(A1) |
申请公布日期 |
2009.11.18 |
申请号 |
EP20090159782 |
申请日期 |
2009.05.08 |
申请人 |
JCR PHARMACEUTICALS CO., LTD. |
发明人 |
SUGIMURA, ATSUSHI;DAIMON, KATSUYA;MIHARA, KAZUTOSHI;ITO, YAE |
分类号 |
C07K14/59 |
主分类号 |
C07K14/59 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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