摘要 |
A method for extracting anticancer substance from the microorganism is provided to obtain the anticancer substance showing strong toxicity against the cancer cell such as leukemia of human body, measured by the biological activity experiment. The method for extracting anticancer substance comprises the steps of: obtaining the culture medium of Actinomyces, Streptomyces sp.; removing the mycelia from the culture medium of Streptomyces sp. to obtain the filtered solution of culture medium; extracting the filtered solution of culture medium with organic solvent and evaporating the organic solvent to obtain the crude extract; fractionating the crude extract according to polarity to obtain the fractions; evaporating and drying the fractions under reduced pressure to obtain polar organic concentrates; and subjecting the polar organic concentrates to chromatography to separate them.
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