| 摘要 |
An object of the present invention is to provide novel ribozyme systems capable of catalyzing tRNA acylation using various carboxylic acids as acyl donors and uses thereof. Disclosed is a ribozyme catalyzing tRNA acylation having a structure consisting of the RNA sequence represented by (formula 1): P1-Z1Z2Z3Z4(N1)1(N1)2 . . . (N1)p-P2-(N2)1(N2)2 . . . (N2)qY1Y2Y3(N3)1(N3)2N4GGN wherein (N1)1-(N1)p each independently represent any monoribonucleotide of U, C, A and G; p represents 3 or 4; (N2)1-(N2)q each independently represent any monoribonucleotide of U, C, A and G; q represents 5 or 6; (N3)1-(N3)2 each independently represent any monoribonucleotide of U, C, A and G; N4 represents any monoribonucleotide of U, C, A and G; Z1-Z4 each independently represent C or G; Y1-Y3 each independently represent C or G; N represents a monoribonucleotide complementary to A or G; and P1 and P2 represent a domain consisting of any RNA sequence capable of having a stem-loop structure.
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