摘要 |
The invention provides media formulations. A complete media formulation comprising the following components: albumin; an iron carrier; glutamine; a glycosidase or hydrolase and/or a glycosaminoglycan degradation product; fibroblast growth factor (FGF); a salt or mineral; and essential amino acids, at an osmolarity of about 220-330 mOsm/Litre; and further comprising a globulin. Also are provided methods for culturing cells, including stem cells, in the media formulations. |