Pericarp tissue preferred regulatory region and method of using same

摘要

A Zea mays regulatory region is shown, which provides improved seed preferred, and particularly pericarp preferred expression in plants. Methods of use are also shown in preferentially expressing a heterologous protein to the pericarp tissue of a plant. The sequence is particularly useful in expression of heterologous proteins to the pericarp of monocotyledonous plants, particularly cereals, and maize.

主权项

1. A method for preferentially expressing a nucleic acid molecule in plant seed pericarp tissue, the method comprising:
(a) transforming a plant cell with an expression cassette comprising a regulatory region consisting essentially of SEQ ID NO: 1 or SEQ ID NO: 8; (b) said regulatory region operably linked to said nucleic acid molecule; and (c) detecting transcription of said operably linked nucleic acid molecule to a higher degree in said pericarp of said seed than in endosperm of said seed.