THE USE OF NADPH AND NADH ANALOGS IN THE MEASUREMENT OF ENZYME ACTIVITIES AND METABOLITES

摘要

Kits and methods for measuring enzyme activities and metabolites using NAD H and NADPH analogs are disclosed. The analogs have extended stability in aqueous solutions and can be used as replacements for NADH or NADPH cofactors in analytical procedures. Preferred aspects of the invention include kits containing the NADH and NADPH analogs for use in the measurement of ALT activity, AST activity, Urea , Ammonia, Salicylate, Triglycerides, Pyruvic Acid, Sorbitol Dehydrogenase activity, 5'- Nucleotidase activity, Creatine Kinase activity, 2,3-Diphosphoglyceric Acid, Adenosine 5'-triphosphate, .alpha.-Hydroxybutyrate Dehydrogenase activity, Lactate Dehydrogenase activity and the Carbon Dioxide content in analytical samples.