METHODS FOR CAPTURE AND SELECTIVE RELEASE OF NUCLEIC ACIDS USING POLYETHYLENEIMINE AND AN ANIONIC PHOSPHATE ESTER SURFACTANT AND AMPLIFICATION OF SAME

摘要

Nucleic acids can be made available for amplification or other treatment after lysis by contacting the lysate with polyethyleneimine to form a precipitate with the nucleic acids. The nucleic acids are then released from the precipitate by contact with a strong base, and the released nucleic acids are kept in solution with an anionic phosphate ester surfactant. This method for preparing specimen samples is simple and quite rapid.