Method for preparing a monoclonal antibody to the common epitope of nonstructural NSs protein of watermelon silver mottle virus and assay for detection of serological related species in the genus tospovirus with the monoclonal antibody

摘要

The invention is an assay for detection of Watermelon silver mottle virus (WSMoV)-serogroup tospoviruses using a monoclonal antibody and a method for preparing the monoclonal antibody. A hybridoma cell line that produces a monoclonal antibody against the NSs proteins of WSMoV-serogroup tospoviruses was produced. The hybridoma cell line produces a monoclonal antibody binding with peptide SEQ ID No. 19.