| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide an improved method for forming a cultured multilayer epidermal sheet using a dried amnion produced by a specific drying method. <P>SOLUTION: The amnion itself, dried while holding the cell texture of the raw amnion by repeating for several times a pressure-reducing operation of continuously heating the human amnion placed in a treating vessel by a far infrared heater installed in the treating vessel, and forming the interior of the treating vessel into a pressure-reduced state, and a pressure-returning operation of returning the pressure in the treating vessel in the pressure-reduced state while also irradiating the raw amnion with a microwave from a microwave heating device installed in the exterior of the treating vessel to heat the amnion has functions of a supporter (scaffold) and a supporting cell (feeder cell). The formation of the multilayer sheet of the epidermal cells using the dried amnion can be carried out in the absence of components derived from a foreign animal in a culture liquid. <P>COPYRIGHT: (C)2010,JPO&INPIT |
