KIT FOR DETECTING MYCOBACTERIUM LEPRAE AND DETECTION METHOD THEREOF

摘要

PURPOSE: A kit and a method for diagnosing leprosy are provided to detect Mycobacterium leprae with improved sensitivity and specificity. CONSTITUTION: A kit for diagnosing leprosy comprises a tube containing R1(5'-CGGGGTAGGGGCGTTTTAGT-3'; sequence number 1), R2(5'-CTAGAAGGTTGCCGTATGTG-3'; sequence number 2), R3(5'-GCGTTTTAGTGTGCATGTCA-3'; sequence number 3), and R4(5'-GGATCATCGATGCACTGTTC-3'; sequence number 4) primers. A method for diagnosing leprosy comprises: a step of preparing DNA sample from an animal; a step of performing PCR using R1(5'-CGGGGTAGGGGCGTTTTAGT-3';sequence number 1), R2(5'-CTAGAAGGTTGCCGTATGTG-3';sequence number 2), R3(5'-GCGTTTTAGTGTGCATGTCA-3';sequence number 3) and R4(5'-GGATCATCGATGCACTGTTC-3';sequence number 4); and a step of isolating amplified PCR product.