| 摘要 |
The present invention refers to the design, construction, characterisation and expression of 4 variant peptides (immunogens) with a lower toxicity of the native toxin CssII from the venom of scorpions of the genus Centruroides suffuses suffuses, which affects sodium channels in mammals; fragments of DNA encoding the said toxin; genetic constructions comprising DNA fragments encoding said immunogens; and the uses thereof as an immunogene for the production of non-human mammals, including horses, antibodies against the total soluble venom of scorpions of the genus Centruroides; also referring to serotherapeutic and antivenom agents obtained from said antibodies. The invention further relates to expression vectors comprising the DNA fragments encoding the recombinant immunogens of the present invention, the recombinant host cells which comprise such vectors, as well as the methods for the expression thereof.
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