The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.
申请公布号
CA2721582(A1)
申请公布日期
2009.10.29
申请号
CA20092721582
申请日期
2009.04.17
申请人
BIO-RAD LABORATORIES, INC.
发明人
WATKINS, MICHAEL I.;MARR, GREGORY A.;YANG, XIAOYUN;BRUEHL, RICHARD;SHAN, DAMING;COLEMAN, PATRICK F.