3' Biased Detection of Nucleic Acids

摘要

The invention provides materials and methods for the detection of nucleic acid expression via the 3' portion of expressed sequences. Embodiments of the invention include the use of microarrays comprising nucleic acid probes that are complementary to the 3' end of expressed sequences and by the use of quantitative PCR (Q-PCR) based amplification of sequences found at or near the 3' end of expressed sequences. The invention may be used to detect the presence of expressed nucleic acids encoding particular gene products (sequences present in a "transcriptome").