| 摘要 |
<p>The present teachings pertain to methods, compositions, reaction mixtures, and kits for mapping a low complexity sequence to a iocus in a genome. In some embodiments, the low complexity sequence can be used to determine the methySation profile of a target nucleic acid. A strand-replacing reaction results in a product containing a first strand and a second strand, which can be connected together with a stem-loop adapter to form a single strand. A sequencing reaction can compare the two strands of the product, allowing the experimentalist to both map the sequence to a locus in a reference genome, as well as ascertain the methylation profile of the original target nucleic acid.</p> |
