发明名称 |
IMMUNOASSAY FOR SPECIFIC DETERMINATION OF S-ADENOSYMETHIONINE AND ANALOGS THEREOF IN BIOLOGICAL SAMPLES |
摘要 |
<p>This invention pertains to a method for detecting a compound in the presence of other compounds that are substantially similar in structure and metabolically related to the analyte. The invention is particularly suited for the detection of S-adenosylmethionine in the presence of S-adenosylhomocysteine, other nucleosides and derivatives in a biological sample. The methods of this invention involve an antibody produced specifically against S-adenosylmethionine; particularly, analogs modified strategically at the sulfonium position. An assay protocol comprises chemically modified analyte analog linked to an enzymatic reporter and the aforementioned antibody was used to demonstrate the assay specificity and sensitivity. Additional assay method with immobilized immunogen, the specific antibody, and an enzyme labeled secondary antibody was also described for illustration. The invention also features hapten design and novel compounds used as haptens to prepare immunogen and for the specific antibody production.</p> |
申请公布号 |
WO2009131643(A1) |
申请公布日期 |
2009.10.29 |
申请号 |
WO2009US02398 |
申请日期 |
2009.04.17 |
申请人 |
MONABIO, INC.;CHANG, CHIU, CHIN |
发明人 |
CHANG, CHIU, CHIN |
分类号 |
A61K39/395;C07K17/14;C12P21/04 |
主分类号 |
A61K39/395 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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