Fluorescence measurement method utilizing surface tension apparatus

摘要

Apparatus and method for measuring the fluorescence of nanodrop liquid samples is described in which the sample is held by surfa tension between two anvil surfaces (20, 24). Each anvil surface (20, 24) has an embedded optical fiber (18) with its end finished fl with the surface in the containment area wetted by the sample with the fiber (18) in line. Sample excitation is provided from the sid of the sample remote from the containment area. By selection of the fiber transmission numeric aperture the impact of exciting and ambient light on the measurement is minimized. A method of virtual filtering is taught in which any ambient or exciting light that does impinge on the measuring sensor is corrected by subtracting a scaled representation of the source from the measurement. The method and apparatus is capable of detecting (1) femptomole of sodium fluorescein in (1) microliter of TE buffer.