Method for automated high throughput identification of carbohydrates and carbohydrate mixture composition patterns as well as systems therefore

摘要

The present invention relates to methods for the identification of compounds in carbohydrate mixture compositions as well as the determination of carbohydrate mixture composition patterns, based on determining migration time (indices) using capillary gel electrophoresis- laser induced fluorescence and identifying said carbohydrate components based on comparing said migration time (indices) with standard migration time (indices) from a database. In a further aspect, the present invention relates to a method for carbohydrate mixture composition pattern profiling, like glycosylation pattern profiling using capillary gel electrophoresis- laser induced fluorescence (CGE-LIF). In another aspect, the present invention refers to a system for an automated determination and/or identification of carbohydrates and/or carbohydrate mixture composition patterns (e.g.: glycosylation patterns). Finally, the present invention relates to a database containing normalized migration times and/or migration time indices of carbohydrates.