GENOME-WIDE CONSTRUCTION OF SCHIZOSACCHAROMYCES POMBE HETEROZYGOUS DELETION MUTANTS CONTAINING GENE-SPECIFIC BARCODES BY THE METHODS OF 4-ROUND SERIAL OR BLOCK PCR, OR TOTAL GENE SYNTHESIS THEREOF

摘要

PURPOSE: A method for producing gene targeting heterozygote fission yeast strain is provided to detect drug active point at the genetic level. CONSTITUTION: A method for producing gene targeting heterozygote fission yeast strain containing a strain-specific bar code comprises gene synthesis, 4-round serial PCR, and 4-round block PCR. In the method, a selection marker gene, gene-specific microarray bar code sequence, and gene targeting deletion cassette.